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Confocal microscopes

Leica SP8

LeicaSP8

The centre's newly acquired Leica TCS SP8 confocal has been designed with optimal photon efficiency and high speed.  This system has 4 detectors (2 GaAsP and 2 PMT) and a motorized xyz-stage.

Objectives: 10x 0.4, 20x 0.75, 40x 1.3 (oil), 63x 1.4 (oil), 63x 1.2 water

laser lines:

7 laser lines (405, 458, 488, 496, 514, 568, and 633) 

  • 2 chilled PMT fluorescence detectors (digital spectral definition in 1 nm increments) plus one transmitted light detector, 8- or 12-bit output, 16-bit possible in galvo mode.
  • 2 high sensitivity, low read noise hybrid GaAsP detector HyD with photon counting mode.
  • xy scanning stage for tiling and multi-point sampling.
  • z-Galvo stage for precision Z-sectioning.
  • Inverted platform for imaging on slides or live cell dishes.
  • AOBS (acousto-optical beam splitter) plus sequential scanning capability allows for rapid sequential scanning of fluorophores with minimal bleed-through or cross-talk.

Leica SP2

The Leica TCS SP2 is a point scanning confocal microscope, based around an inverted DMIRE stand. The system has three photomultipliers for fluorescence imaging with an additional one for transmitted light. It can allow users a wide choice of fluorochrome dyes including: DAPI, FITC, TRITC, TEXAS RED and CY5. Live-cell experimentation on this system is possible through the use of a stage-top live-cell incubation chamber where both temperature and CO2 concentration can be set and monitored.

A unique feature of the system is the ability to spectrally scan a specimen’s emission wavelength at a given laser excitation line, and thus determine whether the signal originates from the fluorochrome used or from background autofluorescence or bleethrough.

The operating software offers FRAP and FRET with built in ‘Wizards’ to help new users.

Laser lines

  • Argon UV, 405nm
  • Multi line Argon; 458nm, 476nm, 488nm, 514nm
  • HeNe; 543nm
  • HeNe; 633nm

Imaging modes

  • Volume x,y,z (3D)
  • time lapse x,y,z,t (4D)
  • live-cell

Objectives

Confocal sp2 invert table

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