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Evaluation of the performance of the lab-built STED microscope from ideal calibration samples. (a) Estimation of the resolution of the confocal/STED modality from individual antibody cluster (dye STAR 635P). The resolution enhancement is on the order of 3. (b) Performance of the STED modality in a case of a dense sample formed by 100nm beads. (c) Confocal/STED images of amyloid fibrils labelled with the dye STAR 635P.

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