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Time-lapse imaging of Zebrafish optic tract development

This is a time-lapse imaging of a 55 hours post-fertilization transgenic zebrafish embryo during 24 hours. Here RFP expression is driven under the control of atoh7 promoter, labeling all retinal ganglion cells (RGCs) in the retina. We can clearly observe RGC axons projecting out of the retina inside the brain and finally reaching their target in the tectum, where they start to branch and make synapses. The researchers aim to study the molecular mechanisms regulating these events by combining genetic approaches and long-term high resolution imaging.