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Excitation:

Omicron laser combiner with 5 possible excitation wavelengths

  • 445 nm for CFP
  • 488 nm for GFP, YFP
  • 515 nm YFP
  • 561 nm for mCherry, TagRFP, DsRed
  • 638 nm
  • Multi-Photon excitation (690-1000 nm)

 

Emission:

  • 460-500 nm (480/40)
  • 500-550 nm (525/50)
  • 525-565 nm (545/40) 
  • 570-640 nm (605/70)
  • 620-695 nm (670/50)

in addition custom filters can be used if necessary

Detection: 

Hamamatsu Orca Flash 4 camera with up to 50 planes/sec

 

Specifics:

  • Adaption of excitation volume (light sheet length) to different samples with tunable lenses
  • Heated sample holder