Inverted Structured Illumination Microscope
Model: Zeiss Elyra 7 Lattice SIM
Status: Operational
The system includes
- 63x oil immersion lens (1.4 and 1.46 NA)
- Four high power visible CW laser lines (405, 488, 561 and 642 nm)
- 2 fast and efficient sCMOS cameras.
- Automated stage for multi-position imaging and tiling.
- Incubation system for temperature, humidity and CO2 control.
Key Specifications
- Lateral resolution (XY): 120 nm
- Axial resolution (Z): 300 nm
- 17 SIM image frames per second at 512 × 512 resolution and 1 ms exposure time (15 phase images per one SIM image)
- Simultaneous capture of two channels (filters cubes user for sequential imaging of more channels)
Brief sample preparation notes:
- SIM operates using typical fluorescence mechanism. Samples prepared as you would for an inverted confocal microscope are suitable
- SIM resolution relies on projecting very high contrast patterns which sample thickness can perturb. Typical sample thickness for high quality data ~ 10-20 micrometres on imaging quality glass coverslips (#1.5).
Training requests can be made through our online booking system