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Examples of Use

Single Color Drosophila Active Zone

Single Color STED imaging

STED and confocal images of ‘active zones’ at the Drosophila larval neuromuscular junction. Visualised with an antibody (NC82) against the active zone protein Bruchpilot. Labelled with Abberior STAR RED. Images taken in collaboration with Matthias Landgraf, Dept. of Zoology.

STEDActiveZoneZoom

STEDExampleMatthias

 

 

 

 

Single color STED spermatocytes

 

Drosophila primary spermatocyte nucleus labeled with anti-histone (STAR Red) showing the organization of chromatin blocks (chromosomes) and, at higher power, the structure of individual chromatin fibres.

Comparison of standard confocal imaging (left panels) and STED imaging (right panels)

Images taken in collaboration with Rob White, Department of Physiology, Development and Neuroscience.

Multi color STED spermatocytes

Multi Colour STED imaging

 

Two-colour labeling of Drosophila primary spermatocyte nucleus labeled with red: anti-histone (STAR Red) and green: active RNA Polymerase (STAR-580). The double labeling shows the arrangement of transcriptionally-active chromatin.

Comparison of standard confocal imaging (left panel) and STED imaging (right panel)

Images taken in collaboration with Rob White, Department of Physiology, Development and Neuroscience.