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Example of two photon time lapse light sheet imaging

The movie shows a Ath5:gapRFP transgenic zebrafish retina. In this transgenic, the Ath5 promotor drives the expression of membrane tagged RFP in retinal ganglion cells as well as in photoreceptors, horizontal and amacrine cells. We can observe the axons of retinal ganglion cells exiting the eye and projecting towards the brain.

Shown are maximum intensity projections in three directions based on imaging stacks of ~ 300um x 300um x 200um stacks. The imaging was performed using 927 nm excitation every 2.5 min for more than 8 hours.

Project in collaboration with Xana Almeida, Harris group