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Confocal microscopes

Leica SP8 (Core)

Leica SP8 Core

The centre's newly acquired Leica TCS SP8 confocal has been designed with optimal photon efficiency and high speed.  This system has 3 detectors (1 sensitive Hybrid GaAsP and 2 PMT) and a motorized xyz-stage.

Objectives: 10x 0.4, 20x 0.75, 40x 1.3 (oil), 63x 1.4 (oil)

laser lines:

4 laser lines (405, 488, 552, and 638) 

  • 2 chilled PMT fluorescence detectors (digital spectral definition in 1 nm increments) plus one transmitted light detector, 8- or 12-bit output, 16-bit possible in galvo mode.
  • 1 high sensitivity, low read noise hybrid GaAsP detector HyD with photon counting mode.
  • xy scanning stage for tiling and multi-point sampling.
  • Inverted platform for imaging on slides or live cell dishes.

Book SP8 confocal core (PPMS login required)

 

Leica SP8 (Advanced with FRAP)

The centre's Leica TCS SP8 confocal has been designed with optimal photon efficiency and high speed.  This system has 4 detectors (2 GaAsP and 2 PMT) and a motorized xyz-stage.

Objectives: 10x 0.4, 20x 0.75, 40x 1.3 (oil), 63x 1.4 (oil)

laser lines:

7 laser lines (405, 458, 488, 496, 514, 561, and 633) 

  • 2 chilled PMT fluorescence detectors (digital spectral definition in 1 nm increments) plus one transmitted light detector, 8- or 12-bit output, 16-bit possible in galvo mode.
  • 2 high sensitivity, low read noise hybrid GaAsP detector HyD with photon counting mode.
  • Optional resonant scanner for fast imaging
  • xy scanning stage for tiling and multi-point sampling.
  • z-Galvo stage for precision Z-sectioning.
  • Inverted platform for imaging on slides or live cell dishes.
  • AOBS (acousto-optical beam splitter) plus sequential scanning capability allows for rapid sequential scanning of fluorophores with minimal bleed-through or cross-talk.

Book SP8 confocal advanced (PPMS login required)

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